laser gene megalign program Search Results


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DNASTAR laser gene megalign program
Laser Gene Megalign Program, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Novus Biologicals anti megalin antibody
Figure 1. Expression and localization of the hemagglutinin angiotensin II type 1 receptor- associated protein (HA-ATRAP) transgene in renal ATRAP transgenic (Tg) mice. A, Representative Western blot analysis of the HA-ATRAP transgene with polyclonal anti- HA antibody. B, Representative Western blot analysis of the total ATRAP protein expression in the kidney of wild-type (Wt) and Tg mice. C, Renal cortical section showing expression of the HA-ATRAP transgene in renal tubules detected by anti-HA antibody (top left). Consecutive sections showing total ATRAP protein expression (transgene HA-ATRAP and endogenous ATRAP) detected by anti-ATRAP antibody (top center). Consecutive sections were also stained with a monoclonal antibody against <t>megalin</t> (top right), a specific marker of proximal tubules, a monoclonal antibody <t>against</t> <t>calbindin-D</t> (bottom left), a specific marker of distal convoluted tubules (DCT) and connecting tubules (CNT), and a polyclonal antibody against aquaporin-2 (AQP2; bottom right), a specific marker of collecting ducts. Original magnification, ×100. D, Representative image of a hematoxylin/eosin-stained section of the proximal (bottom left) and distal (bottom right) tubules in the renal cortex before and after laser microdissection. Original magnification, ×400. Quantitative analysis (top) of mRNA expression in the proximal and distal tubules of the renal cortex. Values are calculated relative to those obtained for ATRAP mRNA expression in extracts from proximal tubules of Wt mice and are expressed as the mean (n=4 in each group).
Anti Megalin Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti megalin antibody - by Bioz Stars, 2026-10
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DNASTAR megalign dnastar laser gene 7
Figure 1. Expression and localization of the hemagglutinin angiotensin II type 1 receptor- associated protein (HA-ATRAP) transgene in renal ATRAP transgenic (Tg) mice. A, Representative Western blot analysis of the HA-ATRAP transgene with polyclonal anti- HA antibody. B, Representative Western blot analysis of the total ATRAP protein expression in the kidney of wild-type (Wt) and Tg mice. C, Renal cortical section showing expression of the HA-ATRAP transgene in renal tubules detected by anti-HA antibody (top left). Consecutive sections showing total ATRAP protein expression (transgene HA-ATRAP and endogenous ATRAP) detected by anti-ATRAP antibody (top center). Consecutive sections were also stained with a monoclonal antibody against <t>megalin</t> (top right), a specific marker of proximal tubules, a monoclonal antibody <t>against</t> <t>calbindin-D</t> (bottom left), a specific marker of distal convoluted tubules (DCT) and connecting tubules (CNT), and a polyclonal antibody against aquaporin-2 (AQP2; bottom right), a specific marker of collecting ducts. Original magnification, ×100. D, Representative image of a hematoxylin/eosin-stained section of the proximal (bottom left) and distal (bottom right) tubules in the renal cortex before and after laser microdissection. Original magnification, ×400. Quantitative analysis (top) of mRNA expression in the proximal and distal tubules of the renal cortex. Values are calculated relative to those obtained for ATRAP mRNA expression in extracts from proximal tubules of Wt mice and are expressed as the mean (n=4 in each group).
Megalign Dnastar Laser Gene 7, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. Expression and localization of the hemagglutinin angiotensin II type 1 receptor- associated protein (HA-ATRAP) transgene in renal ATRAP transgenic (Tg) mice. A, Representative Western blot analysis of the HA-ATRAP transgene with polyclonal anti- HA antibody. B, Representative Western blot analysis of the total ATRAP protein expression in the kidney of wild-type (Wt) and Tg mice. C, Renal cortical section showing expression of the HA-ATRAP transgene in renal tubules detected by anti-HA antibody (top left). Consecutive sections showing total ATRAP protein expression (transgene HA-ATRAP and endogenous ATRAP) detected by anti-ATRAP antibody (top center). Consecutive sections were also stained with a monoclonal antibody against megalin (top right), a specific marker of proximal tubules, a monoclonal antibody against calbindin-D (bottom left), a specific marker of distal convoluted tubules (DCT) and connecting tubules (CNT), and a polyclonal antibody against aquaporin-2 (AQP2; bottom right), a specific marker of collecting ducts. Original magnification, ×100. D, Representative image of a hematoxylin/eosin-stained section of the proximal (bottom left) and distal (bottom right) tubules in the renal cortex before and after laser microdissection. Original magnification, ×400. Quantitative analysis (top) of mRNA expression in the proximal and distal tubules of the renal cortex. Values are calculated relative to those obtained for ATRAP mRNA expression in extracts from proximal tubules of Wt mice and are expressed as the mean (n=4 in each group).

Journal: Hypertension

Article Title: Enhanced Angiotensin Receptor-Associated Protein in Renal Tubule Suppresses Angiotensin-Dependent Hypertension

doi: 10.1161/hypertensionaha.111.00572

Figure Lengend Snippet: Figure 1. Expression and localization of the hemagglutinin angiotensin II type 1 receptor- associated protein (HA-ATRAP) transgene in renal ATRAP transgenic (Tg) mice. A, Representative Western blot analysis of the HA-ATRAP transgene with polyclonal anti- HA antibody. B, Representative Western blot analysis of the total ATRAP protein expression in the kidney of wild-type (Wt) and Tg mice. C, Renal cortical section showing expression of the HA-ATRAP transgene in renal tubules detected by anti-HA antibody (top left). Consecutive sections showing total ATRAP protein expression (transgene HA-ATRAP and endogenous ATRAP) detected by anti-ATRAP antibody (top center). Consecutive sections were also stained with a monoclonal antibody against megalin (top right), a specific marker of proximal tubules, a monoclonal antibody against calbindin-D (bottom left), a specific marker of distal convoluted tubules (DCT) and connecting tubules (CNT), and a polyclonal antibody against aquaporin-2 (AQP2; bottom right), a specific marker of collecting ducts. Original magnification, ×100. D, Representative image of a hematoxylin/eosin-stained section of the proximal (bottom left) and distal (bottom right) tubules in the renal cortex before and after laser microdissection. Original magnification, ×400. Quantitative analysis (top) of mRNA expression in the proximal and distal tubules of the renal cortex. Values are calculated relative to those obtained for ATRAP mRNA expression in extracts from proximal tubules of Wt mice and are expressed as the mean (n=4 in each group).

Article Snippet: The sections were then incubated with one of the following: 1) anti-HA antibody diluted at 1:100, 2) anti-ATRAP antibody diluted at 1:100, 3) anti-aquaporin 2 antibody (254-271, CALBIOCHEM) diluted at 1:200, 4) anti-calbindin D-28K antibody (C9848, Sigma-Aldrich) diluted at 1:3000, or 5) anti-megalin antibody (NB110-96417, Novus Biologicals) diluted at 1:1000.

Techniques: Expressing, Transgenic Assay, Western Blot, Staining, Marker, Laser Capture Microdissection